目的 筛选诱导降香檀结香的活性真菌。方法 野外条件下,将25株真菌固体菌种分别接种于正常生长的6~7年生降香檀树干上,通过物理表观性状和化学质量初步分析真菌诱导降香檀形成的降香材料,采用分光光度法测定诱导形成降香材料中总黄酮的含量,采用热浸法测定降香醇溶性浸出物的含量。结果 筛选到6株可诱导降香檀形成降香的活性真菌。结论 本实验结果具有重要的理论意义和实际应用价值。
Abstract
OBJECTIVE To select the fungi which can induce Dalbergia odorifera to produce active substances. METHODS Twenty-five fungi were inoculated in the stems of six or seven-year-old D. odorifera under natural conditions. Fungi with biological activity to induce D. odorifera producing active substances were obtained through field screening and chemical quality evaluation. The ethanol-soluble extractives and total flavonoids of D. odorifera were analyzed by spectrophotometry and hot maceration method, respectively. RESULTS Six active fungi inducing the formation of heartwood were obtained. CONCLUSION This study is very important for utilizing fungi in the induction of D. odorifera and sustainable utilization of this special medicinal material.
关键词
降香檀 /
降香 /
真菌 /
总黄酮 /
醇溶性浸出物
{{custom_keyword}} /
Key words
Dalbergia odorifera /
active substance /
fungi /
total flavonoid /
ethanol-soluble extractives
{{custom_keyword}} /
中图分类号:
R282
{{custom_clc.code}}
({{custom_clc.text}})
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] CHEN H Y. Hainan Flora (海南植物志)[M]. Beijing:Science Press,1964:289-290.
[2] Ch. P(2010)Vol I (中国药典 2010 年版 . 一部) [S].2010:213,appendix 62.
[3] WEI J H, MENG H, FENG J D, et al. The methods which inducing the active substance from Dalbergia odorifera: China, CN 101663969 A. 5 [P]. 2010-03-10.
[4] Guangdong Institute of Plant. Preliminary uncovering the secret of agarwood formation[J]. Acta Bot Sin(植物学报),1976,18(4):287-291.
[5] FENG N X. Preliminary Study on Endophytic Fungi of Aquilaria sinensis[D]. Nanchang : Nanchang University, 2008.
[6] XU W N. Evaluation on Key Technology of Fungi Infection-induced Aloes-forming Effect and Preliminary Research on the Mechanism of the Eaglewood Formation[D]. Guangzhou: Guangdong Pharmaceutical University,2011.
[7] CUI J L,GUO S X,FU S B,et al. Effects of inoculating fungi on agilawood formation in Aquilaria sinensis[J]. Chin Sci Bull,2013,58(66):3280-3287.
[8] TAMUFI P,BORUAH P,NATH S C,et al. Fungi from disease agarwood tree (Aquilaria agalocha Roxb. ): two new records[J]. Adv Forest Res,2000,22:182-189.
[9] TABATA Y,WIDJAJA E,MULYANINGSIH T,et al. Structural survey and artificial induction of aloeswood [J]. Wood Res,2003,90(1):11-12.
[10] SUN S S, ZENG X, ZHANG D W , et al. Diverse fungi associated with partial irregular heartwood of Dalbergia odorifera[J]. Sci Rep,2015,5:8464DOI:10. 1038/srep08464.
[11] CUI J L, QIAO P G,GUO S X. Field test and analysis for fungus inducing the formation of agilawood from Aquilaria sinensis[J]. Chin Pharm J (中国药学杂志),2012,47(20) : 1614-1617.
[12] XIAO S E,LIN L. Determination of total flavone content of lignum Dalbergiae odoriferae of various origins[J]. Primary J Chin Mater Med(基层中药杂志),2000,6(14):12-13.
[13] YANG Z H,MEI C, HE X H, et al. Advance in studies on chemical constitutions,pharmacological mechanism and pharmacokinetic profile of Dalbergiae odoriferae Lignum[J]. China J Chin Mater Med(中国中药杂志),2013,38(11):1679-1683.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}